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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in several tumors, which leads to chromosome instability, and so may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant function in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be described. Two supplemental splice variants were being documented but comprehensive nucleotide sequence information is just not nonetheless offered.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded through the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...
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This guide outlines more probable optimization of cellular and immunoassay parameters to make certain the very best success are...
Antibodies bound to the blocking peptide not bind on the epitope over the target protein. By comparing the staining in the blocked antibody compared to the antibody on your own, one can see which staining is distinct.
Focus on Facts Cyclin E belongs into the highly conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation designs which contribute into the temporal coordination of every mitotic event. Cyclin E varieties a fancy with and capabilities being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S phase boundary which is degraded as cells development by way of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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